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KMID : 1007519980070040300
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Food Science and Biotechnology
1998 Volume.7 No. 4 p.300 ~ p.304
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Molecular Cloning of Genes for Lipolytic Enzymes of Aspergillus nidulans
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Lee, Je-Geun
Park, Dae-Ryong/Choi, Eun-Suk/Kim, Woo-Yeon
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Abstract
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Phagemids containing genes coding for lipolytic enzymes were screened from an Aspergillus nidulans cDNA library. The Aspergillus nidulans Uni-ZAP cDNA library was converted into a phagemid library using the ExAssist/SOLR system, which allowed efficient excision of the pBluescript phagemid from the Uni-ZAP vector. SOLR cells harboring pBluescript phagemids with Aspergillus nidulans cDNA inserts were plated on the media containing tributyrin and olive oil with a fluorescent dye, rhodamine B, respectively. Three different cells with lipolytic enzyme activity were isolated based on the formation of both a clear zone and a fluorescent zone. Sizes of the cDNA inserts in three different pBluescript phagemids were found to be 1.3 kb, and 0.6 kb, respectively.
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KEYWORD
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